Bioteknologi Tanah

Penulis

Yudhi Harini Bertham
Soil Science Study Program, Faculty of Agriculture, University of Bengkulu

Sinopsis

Buku ajar Pengantar Bioteknologi Tanah membahas peran penting mikroorganisme tanah dalam mendukung kesuburan tanah, pertumbuhan tanaman, pengelolaan lingkungan, dan pertanian berkelanjutan. Tanah tidak hanya dipandang sebagai media tumbuh, tetapi juga sebagai ekosistem hidup yang dihuni oleh berbagai mikroorganisme bermanfaat, seperti bakteri penambat nitrogen, rizobakteria pemacu pertumbuhan tanaman, fungi mikoriza, serta mikroba perombak bahan pencemar.

Materi dalam buku ini disusun secara sistematis, dimulai dari konsep bioremediasi tanah sebagai teknologi pemanfaatan mikroba untuk menguraikan polutan, dilanjutkan dengan sejarah dan  pengertian dasar bioteknologi. Pembahasan berikutnya menguraikan mikroorganisme penting  dalam bioteknologi tanah, yaitu Rhizobium, Azospirillum, Rhizopseudomonas, Frankia, dan Mikoriza Vesikular Arbuskular. Setiap topik dilengkapi dengan penjelasan mengenai karakteristik mikroorganisme, mekanisme kerja, interaksi dengan tanaman, faktor lingkungan yang memengaruhi aktivitasnya, serta manfaatnya dalam meningkatkan ketersediaan unsur hara dan kesehatan tanaman.

Buku ini juga memberikan dasar pemahaman praktis mengenai isolasi, pengamatan, dan  pemanfaatan mikroorganisme tanah sebagai inokulan hayati. Dengan bahasa yang sederhana namun tetap ilmiah, buku ini diharapkan dapat menjadi bahan ajar bagi mahasiswa pertanian, ilmu tanah, biologi, lingkungan, dan bidang terkait. Kehadiran buku ini diharapkan mampu mendorong mahasiswa untuk memahami, mengembangkan, dan menerapkan bioteknologi tanah secara bijaksana dalam mendukung produktivitas pertanian, efisiensi pemupukan, rehabilitasi lahan, serta pengelolaan lingkungan yang ramah dan berkelanjutan.

Referensi

Abarca, C., Fernandez Bidondo, L., Bompadre, J., & Velázquez, M. S. (2024). Arbuscular mycorrhizal fungi in tomato tolerance to pathogens and nematodes: A comprehensive review. Scientia Horticulturae, 329, 112969. https://doi.org/10.1016/j.scienta.2024.112969

Ahmed, N., Li, J., Li, Y., Deng, L., Deng, L., Chachar, M., Chachar, Z., Chachar, S., Hayat, F., Raza, A., Umrani, J. H., Gong, L., & Tu, P. (2025). Symbiotic synergy: How Arbuscular Mycorrhizal Fungi enhance nutrient uptake, stress tolerance, and soil health through molecular mechanisms and hormonal regulation. IMA Fungus, 16. https://doi.org/10.3897/imafungus.16.144989

Ai, W., Qiu, Y., Hua, J., Chen, Z., Cheng, W., Chen, Y., Zhang, S., Xue, Y., Li, S., Hong, R., Dong, R., & Cao, Y. (2025). Pseudomonas fluorescens P34 colonization impacts expression changes in wheat roots, reshapes rhizosphere microbial communities and promotes wheat plant growth. Microbiological Research, 301, 128306. https://doi.org/10.1016/j.micres.2025.128306

Akansha, J., Thakur, S., Chaithanya, M. S., Gupta, B., Sen, Das, S., Das, B., Rajasekar, N., & Priya, K. (2024). Technological and economic analysis of electrokinetic remediation of contaminated soil: A global perspective and its application in Indian scenario. Heliyon, 10(2), e24293. https://doi.org/10.1016/j.heliyon.2024.e24293

Alattas, H., Glick, B. R., Murphy, D. V., & Scott, C. (2024). Harnessing Pseudomonas spp. for sustainable plant crop protection. Frontiers in Microbiology, 15. https://doi.org/10.3389/fmicb.2024.1485197

An, X.-P., Zhang, Z.-Z., Zhou, L.-J., Feng, H.-D., Sun, R.-T., Hashem, A., Abd-Allah, E. F., & Wu, Q.-S. (2025). Phosphorus absorption by mycorrhizal extraradical hyphae accelerates plant growth and active ingredient production in Polygonum cuspidatum. Chemical and Biological Technologies in Agriculture, 12(1), 123. https://doi.org/10.1186/s40538-025-00844-5

Antunes, P. M., Stürmer, S. L., Bever, J. D., Chagnon, P.-L., Chaudhary, V. B., Deveautour, C., Fahey, C., Kokkoris, V., Lekberg, Y., Powell, J. R., Aguilar-Trigueros, C. A., & Zhang, H. (2025). Enhancing consistency in arbuscular mycorrhizal trait-based research to improve predictions of function. Mycorrhiza, 35(2), 14. https://doi.org/10.1007/s00572-025-01187-7

Aparicio, M. A., Ruiz-Castilla, F. J., Ramos, J., Romera, F. J., & Lucena, C. (2025). The inoculation with Pseudomonas simiae WCS417 strain promotes growth and the induction of iron-deficiency responses in cucumber plants (Cucumis sativus L.). Planta, 262(6), 136. https://doi.org/10.1007/s00425-025-04844-5

Ashrafi-Saiedlou, S., Rasouli-Sadaghiani, M., Samadi, A., Barin, M., & Sepehr, E. (2024). Aspergillus niger as an eco-friendly agent for potassium release from K-bearing minerals: Isolation, screening and culture medium optimization using Plackett-Burman design and response surface methodology. Heliyon, 10, e29117.

Aslam, Z., Yahya, M., Hussain, H. S., Tabbasum, S., Jalaluddin, S., Khaliq, S., & Yasmin, S. (2024). Development of bacteria-based bioorganic phosphate fertilizer enriched with rock phosphate for sustainable wheat production. Frontiers in Microbiology, 15, 1361574. https://doi.org/10.3389/fmicb.2024.1361574

Aziz, Z. S., Jazza, S. H., Dageem, H. N., Banoon, S. R., Balboul, B. A., & Abdelzaher, M. A. (2024). Bacterial biodegradation of oil-contaminated soil for pollutant abatement contributing to achieve sustainable development goals: A comprehensive review. Results in Engineering, 22, 102083. https://doi.org/10.1016/j.rineng.2024.102083

Bakki, M., Banane, B., Marhane, O., Esmaeel, Q., Hatimi, A., Barka, E. A., Azim, K., & Bouizgarne, B. (2024). Phosphate solubilizing Pseudomonas and Bacillus combined with rock phosphates promoting tomato growth and reducing bacterial canker disease. Frontiers in Microbiology, 15. https://doi.org/10.3389/fmicb.2024.1289466

Bertham, Y. H., Arifin, Z., & Nusantara, A. D. (2019). The Improvement of Yield and Quality of Soybeans in a Coastal Area Using Low Input Technology Based on Biofertilizers. International Journal on Advanced Science, Engineering and Information Technology, 9(3), 787–791. https://doi.org/10.18517/ijaseit.9.3.7614

Bertham, Y. H., Nusantara, A. D., Barchia, F., & Simarmata. (2024). Optimization of upland rice yields inoculated with Azotobacter, Agrobacterium tumefaciens, and Mycorrhizae through the Provision of Humic Acid and Micro-fertilizers in Coastal Land. International Journal of Agricultural Technology, 20(6), 2233–2244.

Bhattacharyya, P. N., Islam, N. F., Sarma, B., Nath, B. C., Al-Ani, L. K. T., & Lesueur, D. (2024). Frankia-actinorhizal symbiosis: a non-chemical biological assemblage for enhanced plant growth, nodulation and reclamation of degraded soils. Symbiosis, 92(1), 1–26. https://doi.org/10.1007/s13199-023-00956-2

Cabanzo-Atilano, I., Sandoval-Villa, M., Almaraz-Suárez, J. J., García-Cué, J. L., Pedraza-Santos, M. E., & Peralta-Sánchez, M. G. (2024). Efficiency of plant growth promoting rhizobacteria (PGPR) in the vegetative development of blackberries

(Rubus spp.) in greenhouse. Chilean Journal of Agricultural Research, 84(1), 70–83. https://doi.org/10.4067/S0718-58392024000100070

Camenzind, T., Aguilar‐Trigueros, C. A., Heuck, M. K., Maerowitz‐McMahan, S., Rillig, M. C., Cornwell, W. K., & Powell, J. R. (2024). Progressing beyond colonization strategies to understand arbuscular mycorrhizal fungal life history. New Phytologist, 244(3), 752–759. https://doi.org/10.1111/nph.20090

Cargill, R. I. M., Shimizu, T. S., Kiers, E. T., & Kokkoris, V. (2025). Cellular anatomy of arbuscular mycorrhizal fungi. Current Biology, 35(11), R545–R562. https://doi.org/10.1016/j.cub.2025.03.053

Chandrasekaran, M. (2024). The role of arbuscular mycorrhizal fungi in refining plant photosynthesis and water status under drought stress: a meta-analysis. Plant, Soil and Environment, 70(8), 502–508. https://doi.org/10.17221/27/2024-PSE

Chatterjee, N., & Margenot, A. J. (2023). Crop growth is increased by arbuscular mycorrhizae for both phosphate rock and soluble phosphorus fertilizers, but fertilizer solubility primarily determines crop growth. Biology and Fertility of Soils, 59(7), 843–862. https://doi.org/10.1007/s00374-023-01751-3

Chen, L., & Liu, Y. (2024). The Function of Root Exudates in the Root Colonization by Beneficial Soil Rhizobacteria. Biology, 13(2), 95. https://doi.org/10.3390/biology13020095

Concawe. (2024). PFAS soil treatment processes: A review of operating ranges and constraints (Report No. 8/24).

Curiel-Alegre, S., Khan, A. H. A., Rad, C., Velasco-Arroyo, B., Rumbo, C., Rivilla, R., Durán, D., Redondo-Nieto, M., Borràs, E., Molognoni, D., Martín-Castellote, S., Juez, B., & Barros, R. (2024). Bioaugmentation and vermicompost facilitated hydrocarbon bioremediation: scaling up from lab to field for petroleum-contaminated soils. Environmental Science and Pollution Research, 32(28), 16601–16616. https://doi.org/10.1007/s11356-024-32916-8

da Silva, G. A., de Assis, D. M. A., Sieverding, E., & Oehl, F. (2024). Four New Families of Arbuscular Mycorrhizal Fungi Within the Order Glomerales. Taxonomy, 4(4), 761–779. https://doi.org/10.3390/taxonomy4040041

de Souza Buzo, F., Garé, L. M., Garcia, N. F. S., de Andrade Silva, M. S. R., Martins, J. T., da Silva, P. H. G., Meireles, F. C.,

de Souza Sales, L. Z., Nogales, A., Rigobelo, E. C., & Arf, O. (2023). Effect of mycorrhizae on phosphate fertilization efficiency and maize growth under field conditions. Scientific Reports, 13(1), 3527. https://doi.org/10.1038/s41598-023-30128-7

Dip, D. P., Sannazzaro, A. I., Otondo, J., Pistorio, M., & Estrella, M. J. (2024). Exploring phosphate solubilizing bacterial communities in rhizospheres of native and exotic forage grasses in alkaline-sodic soils of the flooding pampa. Current Microbiology, 81, 189. https://doi.org/10.1007/s00284-024-03704-x

Dobrzyński, J., Naziębło, A., Kulkova, I., Szpytma, M., Antosik, A., Sitarek-Andrzejczyk, M., & Wróbel, B. (2026). Paenibacillus–Pseudomonas Consortium Improves Barley Performance with Minimal Impact on Native Rhizobacterial Community. Microorganisms, 14(2), 488. https://doi.org/10.3390/microorganisms14020488

Doolotkeldieva, T., Bobusheva, S., & Konurbaeva, M. (2025). The bacterial species’ degradation activities at maximum threshold doses of glyphosate across different pH levels and temperature glyphosate biodegradation by soil bacteria at high doses under variable pH and temperature. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1668968

Ducousso-Detrez, A., Lahrach, Z., Fontaine, J., Lounes-Hadj Sahraoui, A., & Hijri, M. (2024). Cultural techniques capture diverse phosphate-solubilizing bacteria in rock phosphate-enriched habitats. Frontiers in Microbiology, 15, 1280848. https://doi.org/10.3389/fmicb.2024.1280848

Elshafei, A. M., & Mansour, R. (2024). Microbial bioremediation of soils contaminated with petroleum hydrocarbons. Discover Soil, 1(1), 9. https://doi.org/10.1007/s44378-024-00004-5

Etesami, H. (2025). The dual nature of plant growth-promoting bacteria: Benefits, risks, and pathways to sustainable deployment. Current Research in Microbial Sciences, 9, 100421. https://doi.org/10.1016/j.crmicr.2025.100421

Fan, X., Zhu, Y., Jia, Y., Du, P., Wang, W., Liu, J., Lv, Z., Liu, R., & Li, X. (2025). Multi-environment meta-analysis reveals the mechanism of potassium-solubilizing microorganisms in promoting crop yield. Frontiers in Plant Science, 16, 1659478. https://doi.org/10.3389/fpls.2025.1659478

Farhaoui, A., Taoussi, M., Laasli, S.-E., Legrifi, I., El Mazouni, N., Meddich, A., Hijri, M., & Lahlali, R. (2025). Arbuscular mycorrhizal fungi and their role in plant disease control: A state-of-the-art. The Microbe, 8, 100438. https://doi.org/10.1016/j.microb.2025.100438

Farooq, Q. U. A., McComb, J., Hardy, G. E. St. J., & Burgess, T. I. (2024). Soil amendments for management of Phytophthora root rot in avocado and their impact on the soil microbiome. Journal of Plant Pathology, 106(2), 439–455. https://doi.org/10.1007/s42161-024-01604-4

Fendrich, R. C., Silva, M. B., & Marriel, I. E. (2025). Agronomic Efficiency of a New Liquid Inoculant Formulated with a Mixture of Azospirillum brasilense Strains Ab-V5 and Ab-V6 in Corn (Zea mays L.). Microorganisms, 13(10), 2403. https://doi.org/10.3390/microorganisms13102403

Feng, Y., Tian, B., Xiong, J., Lin, G., Cheng, L., Zhang, T., Lin, B., Ke, Z., & Li, X. (2024). Exploring IAA biosynthesis and plant growth promotion mechanism for tomato root endophytes with incomplete IAA synthesis pathways. Chemical and Biological Technologies in Agriculture, 11(1), 187. https://doi.org/10.1186/s40538-024-00712-8

Feng, Z., Liu, X., Qin, Y., Feng, G., Zhou, Y., Zhu, H., & Yao, Q. (2023). Cooperation of arbuscular mycorrhizal fungi and bacteria to facilitate the host plant growth dependent on soil pH. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1116943

Foster, L. R., Yang, J., Riethoven, J.-J. M., Mukhtar, H., & Schachtman, D. P. (2025). Inoculation frequency and maize genotype influence plant growth-promoting effects of soil bacteria under low nitrogen conditions. Frontiers in Plant

Science, 16. https://doi.org/10.3389/fpls.2025.1637156

Ganesh, J., Hewitt, K., Devkota, A. R., Wilson, T., & Kaundal, A. (2024). IAA-producing plant growth promoting rhizobacteria from Ceanothus velutinus enhance cutting propagation efficiency and Arabidopsis biomass. Frontiers in Plant Science, 15. https://doi.org/10.3389/fpls.2024.1374877

Gao, J. N., Xu, M. T., & Uwiringiyimana, E. (2025). Isolation of highly efficient potassium solubilizing bacteria and their effects on nutrient acquisition and growth promotion in tobacco seedlings. BMC Plant Biology, 25, 745. https://doi.org/10.1186/s12870-025-06760-y

Gao, Z., Li, P., Li, C., Tang, R., Wang, M., Chen, J., et al. (2024). Identification, functional annotation, and isolation of phosphorus-solubilizing bacteria in the rhizosphere soil of Swida wilsoniana (Wanger) Sojak. Applied Soil Ecology, 194, 105207. https://doi.org/10.1016/j.apsoil.2023.105207

Gebremeskel, K., Birhane, E., Habtu, S., Haile, M., Chanyalew, S., Tadele, Z., & Assefa, K. (2024). Arbuscular mycorrhizal fungi improve morphological and yield performance of Eragrostis tef genotypes in Tigray, Ethiopia. Scientific Reports, 14(1), 29716. https://doi.org/10.1038/s41598-024-79628-0

Ghorui, M., Chowdhury, S., & Burla, S. (2025). Recent advances in the commercial formulation of arbuscular mycorrhizal inoculants. Frontiers in Industrial Microbiology, 3. https://doi.org/10.3389/finmi.2025.1553472

Giri, B. R., Chattaraj, S., Rath, S., Pattnaik, M. M., Mitra, D., & Thatoi, H. (2025). Unveiling the Molecular Mechanism of Azospirillum in Plant Growth Promotion. Bacteria, 4(3), 36. https://doi.org/10.3390/bacteria4030036

Guan, Y., Bak, F., Hennessy, R. C., Horn Herms, C., Elberg, C. L., Dresbøll, D. B., Winding, A., Sapkota, R., & Nicolaisen, M. H. (2024). The potential of Pseudomonas fluorescens SBW25 to produce viscosin enhances wheat root colonization and shapes root-associated microbial communities in a plant genotype-dependent manner in soil systems. MSphere, 9(7). https://doi.org/10.1128/msphere.00294-24

Helmy, Q., & Kardena, E. (2024). Enhancing field-scale bioremediation of weathered petroleum oil-contaminated soil with biocompost as a bulking agent. Case Studies in Chemical and Environmental Engineering, 9, 100735. https://doi.org/10.1016/j.cscee.2024.100735

Herms, C. H., Hennessy, R. C., Bak, F., Guan, Y., Browne, P. D., Nielsen, T. K., Hansen, L. H., Dresbøll, D. B., & Nicolaisen, M. H. (2025). Pseudomonas taxonomic and functional microdiversity in the wheat rhizosphere is cultivar-dependent and links to disease resistance profile and root diameter. Applied Soil Ecology, 211, 106116. https://doi.org/10.1016/j.apsoil.2025.106116

Hu, B., Flemetakis, E., Liu, Z., Hänsch, R., & Rennenberg, H. (2023). Significance of nitrogen-fixing actinorhizal symbioses for restoration of depleted, degraded, and contaminated soil. Trends in Plant Science, 28(7), 752–764. https://doi.org/10.1016/j.tplants.2023.03.005

Hu, J., Xiang, C., Lu, Y., Jia, M., Feng, Z., & Zhang, S. (2026). Seed coating with plant growth-promoting rhizobacteria enhances potato (Solanum tuberosum L.) growth and yield. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1738090

Hu, X., & Chen, H. (2023). Phosphate solubilizing microorganism: A green measure to effectively control and regulate heavy metal pollution in agricultural soils. Frontiers in Microbiology, 14, 1193670. https://doi.org/10.3389/fmicb.2023.1193670

Ibrahim, N. E., Sevakumaran, V., & Ariffin, F. (2023). Preliminary study on glyphosate-degrading bacteria isolated from agricultural soil. Environmental Advances, 12, 100368. https://doi.org/10.1016/j.envadv.2023.100368

Ikiz, B., Dasgan, H. Y., & Gruda, N. S. (2024). Utilizing the power of plant growth promoting rhizobacteria on reducing mineral fertilizer, improved yield, and nutritional quality of Batavia lettuce in a floating culture. Scientific Reports, 14(1), 1616. https://doi.org/10.1038/s41598-024-51818-w

Jian, P., Zha, Q., Hui, X., Tong, C., & Zhang, D. (2024). Research Progress of Arbuscular Mycorrhizal Fungi Improving Plant Resistance to Temperature Stress. Horticulturae, 10(8), 855. https://doi.org/10.3390/horticulturae10080855

Jian, P., Zhang, H., Xi, X., Yin, X., Sun, P., Zha, Q., & Zhang, D. (2025). Research on the Response of Arbuscular

Mycorrhizae Fungi to Grape Growth Under High Temperature Stress. International Journal of Molecular Sciences, 26(13), 6165. https://doi.org/10.3390/ijms26136165

Kammoun, I., Miotello, G., Slama, K. Ben, Armengaud, J., Ghodhbane-Gtari, F., & Gtari, M. (2024). The impact of Elaeagnus angustifolia root exudates on Parafrankia soli NRRL B-16219 exoproteome. Journal of Genomics, 12, 58–70. https://doi.org/10.7150/jgen.93243

Kaur, R., Kaur, S., Dwibedi, V., Kaur, C., Akhtar, N., & Alzahrani, A. (2023). Development and characterization of rice bran-gum Arabic based encapsulated biofertilizer for enhanced shelf life and controlled bacterial release. Frontiers in Microbiology, 14, 1267730.

Khan, Y., Shah, S., Li, D., & Yang, F. (2025). Arbuscular mycorrhizal fungi-mediated abiotic stress tolerance: Emerging

roles in nutrient exchange, antioxidant defence, and hormonal crosstalk. Plant Stress, 18, 101068. https://doi.org/10.1016/j.stress.2025.101068

Khuong, N. Q., Sakpirom, J., Truong Oanh Oanh, Le Vinh Thuc, Le Thi My Thu, Do Thi Xuan, Le Thanh Quang, & Ly Ngoc Thanh Xuan. (2023). Isolation and characterization of novel potassium-solubilizing purple nonsulfur bacteria from acidic paddy soils using culture-dependent and culture-independent techniques. Brazilian Journal of Microbiology, 54, 2333-2348. https://doi.org/10.1007/s42770-023-01069-0

Kokkoris, V. (2026). Mycelial dynamics in arbuscular mycorrhizal fungi. New Phytologist, 249(2), 691–713. https://doi.org/10.1111/nph.70688

Krett, G., Romsics, C., Jurecska, L., Bódai, V., Erdélyi, B., Márialigeti, K., & Nagymáté, Z. (2024). Field test of a bioaugmentation agent for the bioremediation of chlorinated ethene contaminated sites. Biologia Futura, 75(3), 289–299. https://doi.org/10.1007/s42977-024-00230-6

Kálmán, C. D., Nagy, Z., Berényi, A., Kiss, E., & Posta, K. (2024). Investigating PGPR bacteria for their competence to protect hybrid maize from the factor drought stress. Cereal Research Communications, 52(1), 129–150. https://doi.org/10.1007/s42976-023-00388-0

Lee, S. H., Yun, Y.-B., Kim, D. S., Park, M., Um, Y., & Kang, J. W. (2025). Isolation of plant growth-promoting rhizobacteria from wild-simulated ginseng and evaluation of soil health following its application in the field. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1682016

Lee, S. M., Lee, N., Ryu, S., Song, H. S., Song, S.-H., Kim, Y. J., Chun, S. won, Lee, A. H., & Lee, J. (2026). Coastal marine bacteria with hydrocarbon-degrading capacity: Isolation, screening, and genomic insights. Marine Pollution Bulletin, 225, 119226. https://doi.org/10.1016/j.marpolbul.2026.119226

Li, H., Chen, S., Wang, M., Shi, S., Zhao, W., Xiong, G., et al. (2024). Phosphate solubilization and plant growth properties are promoted by a lactic acid bacterium in calcareous soil. Applied Microbiology and Biotechnology, 108, 24. https://doi.org/10.1007/s00253-023-12850-4

Li, H.-P., Han, Q.-Q., Liu, Q.-M., Gan, Y.-N., Rensing, C., Rivera, W. L., et al. (2023). Roles of phosphate-solubilizing

bacteria in mediating soil legacy phosphorus availability. Microbiological Research, 272, 127375. https://doi.org/10.1016/j.micres.2023.127375

Li, X. L., Lv, X. Y., Ji, J. B., Wang, W. D., Wang, J., Wang, C., et al. (2023). Complete genome sequence of Nguyenibacter sp. L1, a phosphate solubilizing bacterium isolated from Lespedeza bicolor rhizosphere. Frontiers in Microbiology, 14, 1257442. https://doi.org/10.3389/fmicb.2023.1257442

Li, C., Gao, X., Huo, Y., Asseri, T. A. Y., Tian, X., & Luo, K. (2024). Evaluation of biocontrol efficacy of rhizosphere Pseudomonas aeruginosa for management of Phytophthora capsici of pepper. PLOS ONE, 19(9), e0309705. https://doi.org/10.1371/journal.pone.0309705

Liu, H., Ni, B., Duan, A., He, C., & Zhang, J. (2024). High Frankia abundance and low diversity of microbial community are associated with nodulation specificity and stability of sea buckthorn root nodule. Frontiers in Plant Science, 15. https://doi.org/10.3389/fpls.2024.1301447

Liu, X., Yu, J., Wei, J., Qin, Y., Shi, X., Liu, K., Jia, L., & Fan, M. (2025). Inoculation with arbuscular mycorrhizal fungi at the seedling stage of potatoes improves phosphorus use efficiency. Frontiers in Sustainable Food Systems, 9. https://doi.org/10.3389/fsufs.2025.1546032

Liu, C., Han, R., Hu, C.-Y., Deng, S., Liu, X., Chen, Y., et al. (2025). Biogeochemical pathways of phytate-P utilization in soil: Plant and microbial strategies. Environmental Science & Technology, 59, 16069-16089. https://doi.org/10.1021/acs.est.5c00724

Liu, F., Qian, J., Zhu, Y., Wang, P., Hu, J., Lu, B., et al. (2024). Phosphate solubilizing microorganisms increase soil phosphorus availability: A review. Geomicrobiology Journal, 41, 1-16. https://doi.org/10.1080/01490451.2023.2272620

Liu, M., Mooleki, S. P., Li, Y., Schneider, D., Kochian, L. V., & Helgason, B. L. (2025). Phosphorus fertilizer responsive bacteria and fungi in canola (Brassica napus L.) roots are correlated with plant performance. Plant and Soil, 513, 1927-1948. https://doi.org/10.1007/s11104-025-07286-w

Liu, Y., Nessa, A., Zheng, Q., Hu, D., Zhang, W., & Zhang, M. (2023). Inoculations of phosphate-solubilizing bacteria alter soil microbial community and improve phosphorus bioavailability for moso bamboo (Phyllostachys edulis) growth.

Applied Soil Ecology, 189, 104911. https://doi.org/10.1016/j.apsoil.2023.104911

Lucic-Mercy, E., Mercy, L., Jeschke, A., Schneider, C., & Franken, P. (2024). Short-term artificial adaptation of Rhizoglomus irregulare to high phosphate levels and its implications for fungal-plant interactions: phenotypic and transcriptomic insights. Frontiers in Plant Science, 15. https://doi.org/10.3389/fpls.2024.1385245

Lutz, S., Bodenhausen, N., Hess, J., Valzano-Held, A., Waelchli, J., Deslandes-Hérold, G., Schlaeppi, K., & van der Heijden, M. G. A. (2023). Soil microbiome indicators can predict crop growth response to large-scale inoculation with arbuscular mycorrhizal fungi. Nature Microbiology, 8(12), 2277–2289. https://doi.org/10.1038/s41564-023-01520-w

Ma, Q., Chen, H., Yang, Y., & Zhou, B. (2025). Unlocking phosphorus resources: Phosphate-solubilizing microorganisms as a green strategy for activating phosphorus in acidic red soils and promoting crop growth. Frontiers in Microbiology, 16, 1630650. https://doi.org/10.3389/fmicb.2025.1630650

Ma, Y., Chen, S., Liu, S., Guo, L., Zhang, C., Ye, X., & Tian, D. (2025). Phosphate solubilizing fungi enhance insoluble phosphate dissolution via organic acid production: Mechanisms and applications. Frontiers in Microbiology, 16, 1600231. https://doi.org/10.3389/fmicb.2025.1600231

Mbodj, D., Diedhiou, A. G., Manneh, B., Ndiaye, C., Laplaze, L., & Kane, A. (2025). AMF inoculation reduces yield losses in rice exposed to alternate wetting and drying and low fertilization. Scientific Reports, 15(1), 12281. https://doi.org/10.1038/s41598-025-95528-3

Mitra, D., Panneerselvam, P., Senapati, A., Chidambaranathan, P., Nayak, A. K., & Mohapatra, P. K. Das. (2023). Arbuscular Mycorrhizal Fungi Response on Soil Phosphorus Utilization and Enzymes Activities in Aerobic Rice under Phosphorus-Deficient Conditions. Life, 13(5), 1118. https://doi.org/10.3390/life13051118

Mjokwe, S., Elephant, D. E., Manyevere, A., & Mashamaite, C. V. (2025). Unlocking soil phosphorus resources in semi-arid regions using arbuscular mycorrhizal fungi and phosphorus solubilising bacteria: a systematic review. Discover

Soil, 2(1), 105. https://doi.org/10.1007/s44378-025-00137-1

Mohapatra, M., Sahoo, R. K., & Tuteja, N. (2024). Phosphate solubilizing bacteria, Pseudomonas aeruginosa, improve the growth and yield of groundnut (Arachis hypogaea L.). Physiology and Molecular Biology of Plants, 30(7), 1099–1111. https://doi.org/10.1007/s12298-024-01478-x

Montes‐Luz, B., Conrado, A. C., Ellingsen, J. K., Monteiro, R. A., de Souza, E. M., & Stacey, G. (2023). Acetylene Reduction

Assay: A Measure of Nitrogenase Activity in Plants and Bacteria. Current Protocols, 3(5). https://doi.org/10.1002/cpz1.766

Moretti, L. G., Galeriani, T. M., Bossolani, J. W., Portugal, J. R., Jamal, A., Moreira, A., & Crusciol, C. A. C. (2026). Foliar Azospirillum brasilense inoculation and phosphorus application for sustainable maize production in tropical cropping systems. Frontiers in Agronomy, 8. https://doi.org/10.3389/fagro.2026.1731959

Müller, F., Wang, H., Reinhard, A., Omirbekova, A., Berzhanova, R., Mukasheva, T., Urich, T., & Mikolasch, A. (2026). The hydrocarbon-degrading bacteria and fungi in oil contaminated soils of Kazakhstan: microbiome composition, enrichment, isolation and bioremediation potential. Environmental Microbiome, 21(1), 49. https://doi.org/10.1186/s40793-026-00866-y

Nawaz, A., Shahbaz, M., Asadullah, Imran, A., Marghoob, M. U., Imtiaz, M., Mubeen, F., Mubeen, I., Manzoor, M., Muhammad, I., & Chung, G. (2023). Contribution of potassium solubilizing bacteria in improved potassium assimilation and cytosolic K+/Na+ ratio in rice (Oryza sativa L.) under saline-sodic conditions. Frontiers in Microbiology, 14, 1196024. https://doi.org/10.3389/fmicb.2023.1196024

Ng, C. W. W., Yan, W. H., Xia, Y. T., Tsim, K. W. K., & To, J. C. T. (2024). Plant growth-promoting rhizobacteria enhance active ingredient accumulation in medicinal plants at elevated CO2 and are associated with indigenous microbiome. Frontiers in Microbiology, 15. https://doi.org/10.3389/fmicb.2024.1374768

Nie, W., He, Q., Guo, H., Zhang, W., Ma, L., Li, J., & Wen, D. (2024). Arbuscular Mycorrhizal Fungi: Boosting Crop Resilience to Environmental Stresses. Microorganisms, 12(12), 2448. https://doi.org/10.3390/microorganisms12122448

Ocán-Torres, D., Martínez-Burgos, W. J., Manzoki, M. C., Soccol, V. T., Neto, C. J. D., & Soccol, C. R. (2024). Microbial Bioherbicides Based on Cell-Free Phytotoxic Metabolites: Analysis and Perspectives on Their Application in Weed Control as an Innovative Sustainable Solution. Plants, 13(14), 1996. https://doi.org/10.3390/plants13141996

Owiny, A. A., & Dusengemungu, L. (2024). Mycorrhizae in mine wasteland reclamation. Heliyon, 10(13), e33141. https://doi.org/10.1016/j.heliyon.2024.e33141

Papin, M., Polrot, A., Breuil, M.-C., Czarnes, S., Dreux-Zigha, A., Roux, X. Le, Taibi, A., Spor, A., & Philippot, L. (2025). Pre-sowing recurrent inoculation with Pseudomonas fluorescens promotes maize growth. Biology and Fertility of Soils, 61(1), 125–140. https://doi.org/10.1007/s00374-024-01873-2

Park, Y. K., & Chin, Y.-W. (2023). Degradation of Bisphenol A by Bacillus subtilis P74 Isolated from Traditional Fermented Soybean Foods. Microorganisms, 11(9), 2132. https://doi.org/10.3390/microorganisms11092132

Peng, Z., Xing, Y., Ma, Y., Li, S., Jia, Y., Yang, H., & Zhang, F. (2025). Arbuscular mycorrhizal fungi enhance soybean phosphorus uptake and soil fertility under saline-alkaline stress. Scientific Reports, 15(1), 31792. https://doi.org/10.1038/s41598-025-15910-z

Perez-Vazquez, A., Barciela, P., & Prieto, M. A. (2024). In Situ and Ex Situ Bioremediation of Different Persistent Soil Pollutants as Agroecology Tool. Processes, 12(10), 2223. https://doi.org/10.3390/pr12102223

Pranav, P. S., Sivakumar, R., Suvekbala, V., & Rajendhran, J. (2024). Genome-wide identification of root colonization fitness genes in plant growth promoting Pseudomonas asiatica employing transposon-insertion sequencing. Annals of Microbiology, 74(1), 40. https://doi.org/10.1186/s13213-024-01784-5

Ramos Cabrera, E. V., Delgado Espinosa, Z. Y., & Solis Pino, A. F. (2024). Use of phosphorus-solubilizing microorganisms as a biotechnological alternative: A review. Microorganisms, 12(8), 1591. https://doi.org/10.3390/microorganisms12081591

Rasul, M., Yahya, M., Suleman, M., Hakim, S., Mirza, B. S., Mirza, M. S., Reitz, T., Tarkka, M. T., & Yasmin, S. (2024). Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice. Frontiers in Microbiology, 15, 1470019. https://doi.org/10.3389/fmicb.2024.1470019

Richter, F., Calonne-Salmon, M., van der Heijden, M. G. A., Declerck, S., & Stanley, C. E. (2024). AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level. Lab on a Chip, 24(7), 1930–1946. https://doi.org/10.1039/D3LC00859B

Rini, M. V., Irvanto, D., & Ardiyanto, A. (2024). The association of four species of arbuscular mycorrhizal fungi with oil palm seedlings planted on Inceptisol soil from Central Kalimantan Indonesia. International Journal of Agricultural Technology, 20(1), 343–354.

Samal, D. P. K., & Sukla, L. B. (2024). Assessment of potent phosphate-solubilizing bacteria isolated from rice fields in Odisha and unraveling their growth-promoting efficacies. Geomicrobiology Journal, 41, 135-148. https://doi.org/10.1080/01490451.2023.2293735

Santos, F., Melkani, S., Oliveira-Paiva, C., Bini, D., Pavuluri, K., Gatiboni, L., Mahmud, A., Torres, M., McLamore, E., & Bhadha, J. H. (2024). Biofertilizer use in the United States: definition, regulation, and prospects. Applied Microbiology and Biotechnology, 108(1), 511. https://doi.org/10.1007/s00253-024-13347-4

Saxena, P., Barman, M., Verma, S., Das, S., Yadav, J., Kumar, M., Singh, A., Singh, S., Chakdar, H., Saxena, A. K., & Srivastava, A. K. (2026). Potassium-solubilizing bacteria from tropical forest soils enhance potassium and phosphorus uptake in maize. Frontiers in Microbiology, 17, 1788341. https://doi.org/10.3389/fmicb.2026.1788341

Serrano, K., Bezrutczyk, M., Goudeau, D., Dao, T., O’Malley, R., Malmstrom, R. R., Visel, A., Scheller, H. V., & Cole, B. (2024). Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis. Nature Plants, 10(4), 673–688. https://doi.org/10.1038/s41477-024-01666-3

Sharma, A., Kashyap, P. L., & colleagues. (2024). Potassium solubilizing microorganisms as potential biofertilizer: A sustainable climate-resilient approach to improve soil fertility and crop production in agriculture. Journal of Plant Growth Regulation. https://doi.org/10.1007/s00344-024-11297-9

Singh, T., Bisht, N., Ansari, M. M., & Chauhan, P. S. (2024). Pseudomonas putida triggers phosphorus bioavailability and P-transporters under different phosphate regimes to enhance maize growth. Plant Physiology and Biochemistry, 217, 109279. https://doi.org/10.1016/j.plaphy.2024.109279

Skinner, J., Delgado, A. G., Hyman, M., & Chu, M.-Y. J. (2024). Implementation of in situ aerobic cometabolism for groundwater treatment: State of the knowledge and important factors for field operation. Science of The Total Environment, 925, 171667. https://doi.org/10.1016/j.scitotenv.2024.171667

Soliman, E. R. S., Ali, A. M., & Hamada, M. A. (2025). Characterization of Phosphate-Solubilizing Pseudomonas lurida OR400772, and Potential Application in Tricalcium Phosphate Contaminated Soil to Alter Vicia faba Growth, and Gene Expression Patterns. Journal of Soil Science and Plant Nutrition, 25(3), 7890–7908. https://doi.org/10.1007/s42729-025-02639-x

Soumare, A., Sarr, D., & Diedhiou, A. G. (2023). Potassium sources, microorganisms and plant nutrition: Challenges and future research directions. Pedosphere, 33(1), 105-115. https://doi.org/10.1016/j.pedsph.2022.06.025

Sun, W., Shahrajabian, M. H., & Wang, N. (2025). A Study of the Different Strains of the Genus Azospirillum spp. on Increasing Productivity and Stress Resilience in Plants. Plants, 14(2), 267. https://doi.org/10.3390/plants14020267

Sun, X., Tian, X., Jia, M., Hu, X., Zhang, C., & Zhao, L. (2026). Phosphate-solubilizing bacteria: A review of diversity, mechanisms, and applications in sustainable agriculture. Frontiers in Microbiology, 17, 1778470. https://doi.org/10.3389/fmicb.2026.1778470

Ta, Y., Fu, S., Liu, H., Zhang, C., He, M., Yu, H., Ren, Y., Han, Y., Hu, W., Yan, Z., & Wang, Y. (2024). Evaluation of Bacillus velezensis F9 for Cucumber Growth Promotion and Suppression of Fusarium wilt Disease. Microorganisms, 12(9), 1882. https://doi.org/10.3390/microorganisms12091882

Takeuchi, K., Ogiso, M., Ota, A., Nishimura, K., Nishino, C., Omori, Y., Maeda, M., Mizui, R., Yamanaka, H., Ogino, T., &

Seo, S. (2024). Pseudomonas rhodesiae HAI-0804 suppresses Pythium damping off and root rot in cucumber by its efficient root colonization promoted by amendment with glutamate. Frontiers in Microbiology, 15. https://doi.org/10.3389/fmicb.2024.1485167

Tang, B., Man, J., Lehmann, A., & Rillig, M. C. (2024). Arbuscular mycorrhizal fungi attenuate negative impact of drought on soil functions. Global Change Biology, 30(7). https://doi.org/10.1111/gcb.17409

Tang, Y., Wang, L., Fu, J., Zhou, F., Wei, H., Wu, X., Fan, S., & Zhang, X. (2025). Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1538117

Tempestti, J. C. M., Mohan, H., Muthukumar Sathya, P., Lee, S.-W., Venkatachalam, J., Oh, B.-T., & Seralathan, K.-K. (2023). Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge. Environmental Research, 231, 116289. https://doi.org/10.1016/j.envres.2023.116289

Thompson, R. M., George, D., & del Carmen Montero‐Calasanz, M. (2024). Actinorhizal plants and Frankiaceae: The overlooked future of phytoremediation. Environmental Microbiology Reports, 16(6). https://doi.org/10.1111/1758-2229.70033

Tong, A., Liu, W., Liu, X., Zhu, J., Zhou, Y., & Li, J. (2025). Comparative analysis of actinorhizal nodule and associated soil microorganism diversity and structure in three Alnus species. Frontiers in Plant Science, 16. https://doi.org/10.3389/fpls.2025.1572494

Torres-Solórzano, P., Reyes-De la Cruz, H., Altamirano-Hernández, J., Macías-Rodríguez, L., Campos-García, J., & Luna-Cruz, A. (2025). Inoculation with Pseudomonas spp. in Solanum lycopersicum increases yield and fruit quality under nutrient shortage conditions. PeerJ, 13, e19796. https://doi.org/10.7717/peerj.19796

Umer, M., Anwar, N., Mubeen, M., Li, Y., Ali, A., Alshaharni, M. O., & Liu, P. (2025). Roles of arbuscular mycorrhizal fungi in plant growth and disease management for sustainable agriculture. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1616273

Vanitha, T. K., Suresh, G., Bhandi, M. M., Mudiam, M. K. R., & Mohan, S. V. (2023). Microbial degradation of organochlorine pesticide: 2,4-Dichlorophenoxyacetic acid by axenic and mixed consortium. Bioresource Technology, 382, 129031. https://doi.org/10.1016/j.biortech.2023.129031

Wang, H., & Chen, Y. (2024). Protecting plants from pathogens through arbuscular mycorrhiza: Role of fungal diversity. Microbiological Research, 289, 127919. https://doi.org/10.1016/j.micres.2024.127919

Wang, Q., Zhao, J., Glick, B. R., & Tian, J. (2026). Pseudomonas sp. UW4 promotes garlic growth through systemic integration of auxin and ethylene pathways. BMC Plant Biology. https://doi.org/10.1186/s12870-026-08934-8

Wang, C., Pan, G., Lu, X., & Qi, W. (2023). Phosphorus solubilizing microorganisms: Potential promoters of agricultural and environmental engineering. Frontiers in Bioengineering and Biotechnology, 11, 1181078. https://doi.org/10.3389/fbioe.2023.1181078

Wang, T., Sun, Y., Huang, H., Li, Z., Fan, H., Pan, X., Wang, Y., Cao, Y., Wang, K., & Yang, L. (2025). The effect of selected phosphate-solubilizing bacteria on the growth of cotton plants in salinized farmlands. Microorganisms, 13(5), 1075. https://doi.org/10.3390/microorganisms13051075

Wang, Z., Zhao, Y., Shao, J., Wang, J., Xun, W., Sun, X., Xu, Z., Miao, Y., Huang, G., Liu, D., Zhang, R., Shen, Q., & Zhang, N. (2025). Rhizosphere domestication enhances root colonization and plant growth promotion performance of Bacillus velezensis SQR9. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1638130

Wu, Y., Chen, C., & Wang, G. (2024). Inoculation with arbuscular mycorrhizal fungi improves plant biomass and nitrogen and phosphorus nutrients: a meta-analysis. BMC Plant Biology, 24(1), 960. https://doi.org/10.1186/s12870-024-05638-9

Xu, R., Yu, Z., Liu, Y., Liu, Y., Ma, X., Ma, X., Jin, W., Li, Y., Chen, J., & Qin, H. (2025). Soil pH and organic phosphorus co-shape the diversity and assembly processes of arbuscular mycorrhizal fungal community in subtropical broadleaved forests. European Journal of Soil Biology, 127, 103785. https://doi.org/10.1016/j.ejsobi.2025.103785

Yang, J., Lin, S., Li, W., Wang, X., & Li, R. (2025). Biodegradation of p-nitrophenol by Rhodococcus sp. 21391 unveils a two-component p-nitrophenol monooxygenase with broad substrate specificity. Microbial Cell Factories, 24(1), 85. https://doi.org/10.1186/s12934-025-02712-1

Yi, Y., Wang, Y., Liu, W., Zhu, J., Gu, M., Jia, Q., Li, X., Mutalifu, M., Jiang, L., Zhang, W., & Zhang, Z. (2025). Screening, identification, metabolic pathway of di-n-butyl phthalate degrading Priestia megaterium P-7 isolated from long-term film mulched cotton field soil in Xinjiang. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1538746

Yuan, Y., Chen, Z., Huang, X., Wang, F., Guo, H., Huang, Z., & Yang, H. (2023). Comparative analysis of nitrogen content and its influence on actinorhizal nodule and rhizospheric microorganism diversity in three Alnus species. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1230170

Zaman, S. A. U., Bhrdwaj, A., Nayarisseri, A., Khazanehdari, K. A., & Bhuyan, R. (2025). Isolation and characterization of novel hydrocarbon-degrading bacteria from oil polluted soil near Nacharam, Hyderabad, India. Scientific Reports, 15(1), 17219. https://doi.org/10.1038/s41598-025-01081-4

Zhao, S., & Wang, J. (2024). Biodegradation of atrazine and nicosulfuron by Streptomyces nigra LM01: Performance,

degradative pathway, and possible genes involved. Journal of Hazardous Materials, 471, 134336. https://doi.org/10.1016/j.jhazmat.2024.134336

Zhao, Y., Cai, Y., Cao, J., Fan, Y., Yi, Z., Wang, W., & Zuo, W. (2024). Isolation and characterization of potassium-solubilizing rhizobacteria promoting cotton growth in saline-sodic regions. Microorganisms, 12(7), 1474. https://doi.org/10.3390/microorganisms12071474

Zhou, R., Raza, A., Song, J., Janiad, S., Li, Q., Huang, M., & Hassan, M. A. (2025). Growth-promoting effects of arbuscular mycorrhizal fungus Funneliformis mosseae in rice, sesame, sorghum, Egyptian pea and Mexican hat plant. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1549006

Zhu, Y., Xing, Y., Li, Y., Jia, J., Ying, Y., & Shi, W. (2024). The role of phosphate-solubilizing microbial interactions in phosphorus activation and utilization in plant-soil systems: A review. Plants, 13(19), 2686. https://doi.org/10.3390/plants13192686

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